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Glutathione-S-Transferase, Mouse Antibody Peroxidase Conjugated
| Catalogue Number | ANT-220 | |||||||
| Type | Mouse Antibody Monoclonal. | |||||||
| Introduction | GST family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called 'GST gene fusion system'. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's multiple cloning sites results in expression of a fusion protein - the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions. The GST tag has the size of 220 amino acids, which, compared to other tags like the myc- or the FLAG-tag, is quite big. It is fused to the N-terminus of a protein. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. A GST-tag is often used to separate and purify proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins. | |||||||
| Clone | PGSTHRPSHG. | |||||||
| Description | Monoclonal antibodies are produced by immunizing mouse with GST protein. | |||||||
| Ig Subclass | Mouse IgG2b. | |||||||
| Formulation | 1×PBS & 50% glycerol. | |||||||
| Dilution | Western Blotting: 1:1000. | |||||||
| Applications | Western Blot. | |||||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||||
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LY6G FITC ant-099 Rat Anti Mouse LY6G FITC
MBP ant-177 Mouse Anti Maltose-Binding protein
MBP ant-451 Mouse Anti Maltose-Binding protein
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NELL2 ant-030 Mouse Anti Human NEL-Like 2
NFATC1 ant-436 Mouse Anti Human Nuclear factor of activated T-cells cytoplasmic 1
NKp30 ant-402 Mouse Anti Human Natural killer cell p30-related protein
NKp44 ant-401 Mouse Anti Human Natural killer cell p44-related protein
NKp46 ant-303 Mouse Anti Human Natural Cytotoxicity Receptor NKp46
NRAS ant-047 Mouse Anti Human Neuroblastoma RAS Viral Oncogene Homolog
OAT ant-013 Mouse Anti Human Ornithine aminotransferase
p53 ant-192 Recombinant Anti p53 scFv
p53 ant-228 Mouse Anti p53
PAP-1 ant-218 Polyclonal Rabbit Anti Human Pancreatitis-associated protein
PARK7 ant-317 Mouse Anti Human Parkinson Disease Protein 7
PCNA ant-341 Mouse Anti Human Proliferating Cell Nuclear Antigen
PDCD1 ant-417 Mouse Anti Human Programmed Cell Death 1
PDCD12 ant-457 Mouse Anti Human Programmed Cell Death 12
PDCD4 ant-338 Mouse Anti Human Programmed Cell Death 4
PDPN ant-343 Mouse Anti Human Podoplanin
PDX-1 ant-162 Polyclonal Rabbit Anti Pancreas Duodenum Homeobox-1
PGP9.5 ant-335 Mouse Anti Human PGP9.5
PLP ant-200 Mouse Anti Porteolipid Protein of Myelin
PSMA ant-450 Mouse Anti Human Prostate Specific Cancer Antigen
RAB5A ant-337 Mouse Anti Human RAB5A
Rac2 ant-067 Mouse Anti Human Ras-Related C3 Botulinum Toxin Substrate 2
RASSF1A ant-400 Mouse Anti Human Ras association domain-containing protein 1
REXO1 ant-413 Mouse Anti Human RNA exonuclease 1 homolog
SERPINB5 ant-364 Mouse Anti Human Serpin Peptidase Inhibitor, Clade B Member 5
SNAP25 ant-336 Mouse Anti Human Synaptosomal-associated protein 25
SNCA ant-315 Mouse Anti Human Alpha-Synuclein
SNCB ant-454 Polyclonal Rabbit Anti Human Beta-Synuclein
SNCG ant-455 Polyclonal Rabbit Anti Human Gamma-Synuclein
Streptavidin ant-345 Mouse Anti Streptavidin
SUMO2/3 ant-016 Mouse Anti Human Small Ubiquitin-Related Modifier 2/3
TARDBP ant-420 Mouse Anti Human TAR DNA-binding protein 43
TBCA ant-362 Mouse Anti Human Tubulin Folding Cofactor A
Tetanus ant-195 Recombinant Anti-Tetanus Toxoid scFv
TGFBI ant-349 Mouse Anti Human Transforming Growth Factor Beta Induced
TLR2 ant-408 Mouse Anti Human Toll-like receptor 2
TLR7 ant-403 Mouse Anti Human Toll-like receptor 7
TMEFF2 ant-428 Mouse Anti Human Tomoregulin-2
TOLLIP ant-430 Mouse Anti Human Toll-interacting protein
TPD52L1 ant-302 Mouse Anti Human Tumor Protein D52 L1
TREM2 ant-429 Mouse Anti Human Triggering receptor expressed on myeloid cells 2
TTR ant-003 Mouse Anti Human Transthyretin
TUBB2b ant-019 Mouse Anti Human Tubulin beta-2B chain
VAMP2 ant-334 Mouse Anti Human Synaptobrevin-2
VAMP3 ant-352 Mouse Anti Human Synaptobrevin-3
VHL ant-316 Mouse Anti Human Von Hippel-Lindau Protein
Vimentin ant-245 Mouse Anti Human Vimentin
Vimentin Biotin ant-246 Mouse Anti Human Vimentin Biotinylated
Vimentin FITC ant-247 Mouse Anti Human Vimentin FITC
WNT3A ant-431 Mouse Anti Human Protein Wnt-3a
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