品牌	其他品牌	貨號	Y30055
規格	1套	供貨周期	一個月
主要用途	iPS向肝臟細胞定向分化逐步誘導

重慶市華雅干細胞技術有限公司，：

華雅集團旗下授權代理TAKARA Cellartis干細胞產品

圖片為展示效果。實際是3套產品。

iPS向肝臟細胞定向分化試劑盒

產品英文名稱：Cellartis® iPS Cell to Hepatocyte Differentiation System

產品中文名稱：iPS向肝臟細胞定向分化試劑盒

產品介紹

以肝臟細胞為模型的實驗越來越受到重視，在肝臟病變機理研究、藥物代謝研究、藥物毒理評估等方面有廣闊的應用前景。然而，以個體原代肝細胞為材料來源會產生如取材困難、材料批間差大、無法長期穩定供應等一系列難以克服的問題。因此，通過iPS批量轉化生產肝細胞的方法就顯示出特別的優勢。

Cellartis iPS Cell to Hepatocyte Differentiation System可以穩定地、規模化地實現iPS向hepatocyte的誘導轉化生產，而且誘導轉化出的肝細胞能夠穩定表達藥物代謝相關酶系統和藥物轉運系統。

Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系統，客戶既可以對某個特殊病人來源的iPS實現轉化，也可以利用cellartis供應的iPS進行標準化操作。使用本產品的過程起始于誘導人iPS轉化成為確定性內胚層（Definitive Endoderm (DE)）細胞，然后誘導轉化DE至肝細胞。本產品是All-In-One型產品，實驗所需的培養基、基質（coating）等包括在內。應用本產品轉化約3 × 10⁶ hiPS cells,可以獲得約5 × 10⁶ 肝細胞（大約50 cm²面積的單層貼壁細胞）。

利用Cellartis iPS Cell to Hepatocyte Differentiation System轉化得到的肝細胞除了清晰表現出肝細胞的*特征外（如肝細胞特異性標志物、CYP系列酶等），還保留了原始細胞供體者的遺傳基因背景。另外，相比較從組織切除獲得的原代肝臟細胞（通常需要低溫保存），通過本產品系統轉化獲得的肝細胞在更長的時間跨度上保持著生理功能的穩定性。這一點對于毒理學評估和病毒感染評估具有顯著的意義。

產品特點:

高度可重復性*的系統 - *相同的方法已被證明可用于25種不同的iPS細胞系，因此無需針對您的細胞進行優化。

理想的藥物代謝和安全性研究 - 始終如一地生成具有不同遺傳背景的> 90％純的功能性hiPS細胞來源的肝細胞。

定制的起始材料 - 從任何疾病相關的iPS細胞系開始，創建準確的肝臟疾病模型。

準備好個性化醫療 - 為治療應用制造針對患者的疾病特異性細胞。

擴展實驗窗口 - 肝細胞可用于功能測試至少11天。

Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so no need to optimize for your lines.

Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds.

Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models.

Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

產品應用:

iPS向肝臟細胞定向分化

組份：iPS細胞培養基+iPS分化內胚層培養基+內胚層分化肝細胞培養基

每種培養基都是*培養基

Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)：

Cellartis DEF-CS 100 Culture System (Cat. No. Y30020)

? 100 ml DEF-CS 100 Basal Medium

? 800 µl DEF-CS COAT-1 (for 100 ml)

? 300 µl DEF-CS GF-1 (for 100 ml)

? 100 µl DEF-CS GF-2 (for 100 ml)

? 40 µl DEF-CS GF-3 (for 100 ml)

Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030)

? 1 tube Definitive Endoderm Differentiation Coating (10 ml)

? 1 bottle Definitive Endoderm Differentiation Day 0 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 1 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 2 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 3 (25 ml)

? 1 bottle Definitive Endoderm Differentiation Day 4 (47 ml)

? 1 bottle Definitive Endoderm Differentiation Day 6 (47 ml)

Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)

? 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)

? 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)

? 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)

? 1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)

? 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)

? 1 tube Hepatocyte Coating (7.5 ml)

自備試劑：

人多能干細胞

胎牛血清（FBS）或血清替代品（KO-SR）

PBS Dulbecco's（含有Ca2 +和Mg2 +的D-PBS + / +）

PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）

TrypLE選擇酶（1X），不含酚紅

保存：

iPS細胞培養基添加劑–20°C，基礎培養基和包被劑2–8°C

iPS分化內胚層培養基和內胚層分化肝細胞培養基所有組份都保存在–20°C。

Cellartis DEF-CS 100 Culture System (Y30020)：DEF-CS 100 Basal Medium ，COAT-1 2–8°C；GF-1, GF-2, and GF-3 –20°C

Cellartis Definitive Endoderm Differentiation Kit (Y30030)：–20°C

Cellartis Hepatocyte Differentiation Kit (Y30050) ：–20°C

產品性能：

Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

參考文獻：

Asplund, A., et al. (2015) Stem Cell Rev. and Rep. Advance online publication. doi: 10.1007/s12015-015-9621-9

訂購信息

品牌	貨號	產品描述	規格
Cellartis	Y30051	Cellartis® Hepatocyte Maintenance Medium	1(50 mlx2)
Cellartis	Y30055	Cellartis® iPS Cell to Hepatocyte Differentiation System	1 Kit